Trichoderma asperellum has a high antagonistic effect on pepper blight. It can inhibit the activity of Phytophthora through parasitism, metabolite inhibition of sporangia production, and zoospore release, so as to achieve the purpose of preventing and controlling pepper blight.
This post aims to research the control effect of 300 million CFU/g WP of Trichoderma Asperellum on pepper blight and its application technology, so as to provide new excellent products for the biological control of pepper blight.
Field Trial on Vegetable Greenhouse
The experiment was arranged in a plastic greenhouse at the vegetable base in Changshu City, Jiangsu Province. The experimental site is a field where pepper blight often occurs. Transplantation was carried out on May 6, 2020. The pepper seedlings were 50 days old when transplanted, and there were a total of 3,000 peppers.
The soil types, cultivation conditions, and water and fertilizer management in each experimental area were uniform.
Test Method
The pepper was sprayed 15 days after planting. The experiment consisted of 3 treatment groups and 1 clear water control. Each treatment group was repeated 4 times, and the treatment groups were randomly distributed.
The specific treatments are as follows. Treatment groups are
- Trichoderma asperellum WP 3 000 times solution;
- Trichoderma asperellum WP 2 000 times solution;
- Trichoderma harzianum WP 2 000 times solution.
Field Test Investigation and Calculation Method:
We recorded 5 pepper plants in each plot and recorded the number of diseased plants, dead plants, or plants that were obviously wilted.
Experimental Results and Analysis
1. Indoor Pot Experiment
Table 1. Experimental Results of Trichoderma Asperellum Against Pepper Blight | ||||||
Treat | 7 days Later | 14 days Later | 21 days Later | |||
Diseases Index | Efficacy(%) | Diseases Index | Efficacy(%) | Diseases Index | Efficacy(%) | |
Trichoderma asperellum WP 3 000 | 0.32 | 93.0 | 2.40 | 88.0 | 5.56 | 86.5 |
Trichoderma asperellum WP 2 000 | 0.10 | 99.0 | 1.70 | 91.0 | 3.80 | 91.0 |
Trichoderma harzianum WP 2 000 | 0.30 | 92.8 | 2.00 | 90.0 | 8.33 | 80.0 |
CK | 4.90 | 21.00 | 42.5 | |||
2. Field experiment
Table 2 Field effect of Trichoderma Asperellum to Control Pepper Blight | ||||
Treat | 15 Days Later | 30 Days Later | ||
Diseases Index | Efficiency(%) | Diseases Index | Efficiency(%) | |
Trichoderma asperellum 3000 (Root-irrigation) | 0.4 | 93.5 | 3.5 | 85.0 |
Trichoderma asperellum 2000 (Root-irrigation) | 0.2 | 96.5 | 2.6 | 89.0 |
Trichoderma harzianum 2000 (Root-irrigation) | 0.65 | 90.0 | 3.5 | 85.0 |
Trichoderma asperellum 600 Spray | 1.3 | 81.0 | 7.1 | 71.5 |
Trichoderma asperellum 300 Spray | 1.0 | 85.5 | 5.5 | 76.5 |
CK | 7.11 | 25.3 | ||
Discussion
Trichoderma is a biocontrol strain that is safe to crops and highly effective against pathogenic bacteria. It can inhibit or kill pathogenic bacteria through a series of antagonistic effects such as parasitism, production of lytic enzymes, and stimulation of immune responses. Trichoderma conidia can proliferate in large quantities in nature and thus achieve the effect of long-term control of pathogenic bacteria.
Trichoderma asperellum also has a very high survival potential and the ability to adapt to various ecological environments, and can widely exist in soil, plant rhizosphere, and foliage.
The control effect of Trichoderma asperellum on pepper blight is remarkable, and the effective period is more than 30 d. The application method of this biological agent is mainly by irrigating the root or spraying the base of the stem.
Dora Agri supports customizing Trichoderma series formulations. Such as Trichoderma harzianum + Trichoderma asperellum, Trichoderma harzianum + Trichoderma viride.
The combined microorganisms will be much more effective than the single microorganism.