1. Purpose
In order to test the rooting effect on lettuce of Dora Arouse (alginate oligosaccharide) compared with PGRs.
2. Materials & Methods
2.1．Materials
Lettuce seeds、Dora Alginate Oligosaccharide、IBA, NAA.
2.2．Methods
The trial is tested in Changshu National Agricultural Science and Technology Park, Jiangsu Province.
- Group A：Control（CK），
- Group B: IBA（10 ppm），
- Group C: NAA（20ppm），
- Group D: Arouse（5ppm），
- Group E: Arouse (30ppm）;
3. Trial Data Collection
3.1 Lettuce Seeds Germination Rate in 3 Days
Group
Germination rate
A
40%
B
54%
C
70%
D
74%
E
70%
3.2 Lettuce Plant Growth Record on April 12
Plant height in 5 days (cm） | ||||||||||
Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
A | 6.3 | 5.5 | 6.0 | 13.5 | 16.0 | 14.6 | 13.1 | 15.8 | 12.1 | 15.8 |
B | 13.2 | 13.4 | 13.2 | 13.9 | 13.5 | 13.0 | 13.4 | 11.5 | 14.2 | 13.4 |
C | 15.4 | 13.0 | 13.6 | 15.0 | 12.0 | 14.0 | 14.5 | 13.9 | 13.5 | 13.1 |
D | 13.5 | 14.0 | 13.1 | 13.7 | 13.2 | 13.0 | 12.6 | 13.9 | 13.3 | 13.2 |
E | 14.0 | 15.5 | 13.3 | 15.2 | 13.1 | 14.1 | 14.5 | 13.5 | 14.5 | 15.3 |
Root length in 5 days (cm） | ||||||||||
Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
A | 12.4 | 15.1 | 11.4 | 14.7 | 9.0 | 16.5 | 13.4 | 9.7 | 14.1 | 21.2 |
B | 15.5 | 16.2 | 15.8 | 16.8 | 15.7 | 16.0 | 15.9 | 15.4 | 15.0 | 15.4 |
C | 20.7 | 21.6 | 15.7 | 12.4 | 20.5 | 20.6 | 18.9 | 20.3 | 20.4 | 19.2 |
D | 17.3 | 22.5 | 17.1 | 17.5 | 17.8 | 18.1 | 17.7 | 17.1 | 16.8 | 16.8 |
E | 16.5 | 15.7 | 14.2 | 14.0 | 15.2 | 14.2 | 14.1 | 14.1 | 15.2 | 22.2 |
3.3 Lettuce Plant Growth Record on April 23
Plant height in 10 days (cm） | ||||||||||
Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
A | 16.7 | 17.2 | 16.3 | 17.9 | 16.2 | 16.5 | 15.5 | 17.2 | 17.1 | 16.7 |
B | 18.4 | 17.1 | 17.3 | 18.9 | 17.2 | 17.1 | 17.3 | 17.1 | 17.7 | 17.1 |
C | 17.0 | 18.2 | 19.1 | 19.1 | 18.2 | 17.4 | 19.3 | 19.2 | 18.1 | 22.1 |
D | 17.4 | 17.8 | 17.2 | 17.2 | 18.7 | 17.1 | 18.1 | 17.4 | 17.1 | 17.5 |
E | 18.5 | 17.6 | 18.3 | 18.1 | 18.6 | 19.1 | 18.1 | 19.2 | 19.4 | 19.5 |
Root length in 10 days (cm） | ||||||||||
Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
A | 34.2 | 32.0 | 34.8 | 28.2 | 24.0 | 27.0 | 36.0 | 31.1 | 24.2 | 31.4 |
B | 47.5 | 15.0 | 17.0 | 35.4 | 33.6 | 36.0 | 42.3 | 38.0 | 21.2 | 35.4 |
C | 33.4 | 38.7 | 45.2 | 37.2 | 38.3 | 36.2 | 34.8 | 38.2 | 35.4 | 34.6 |
D | 30.4 | 31.6 | 31.1 | 31.4 | 32.2 | 37.4 | 31.7 | 46.0 | 37.4 | 46.8 |
E | 26.7 | 26.2 | 29.2 | 28.2 | 43.8 | 29.2 | 38.2 | 36.2 | 36.2 | 32.4 |
4. Conclusion
Treatment | Germination | Rooting | Growth |
10ppm IBA | 4 | 4 | 4 |
20ppm NAA | 2 | 1 | 1 |
5ppm Arouse | 1 | 2 | 3 |
30ppm Arouse | 2 | 3 | 2 |
*1.2.3.4 in sort to show the effects. | |||
To sum up, 10ppm IBA solution, 20ppm NAA solution, 5ppm, 30ppm alginate oligosaccharide solution can promote the growth of the aerial part of lettuce to a certain extent.
In terms of rooting, 5ppm alginate oligosaccharide and 20ppm NAA solution have obvious rooting-promoting effect, and the root length of lettuce was significantly increased.
As an alginate oligosaccharide extracted from natural seaweed, it only needs 5ppm, the rooting effect can be comparable to that of 20ppm NAA, its effect of promoting germination is also very good, which is more cost-effective and more environmentally friendly.